A series of 5-arylmethylene-pyrimidine -2, 4, 6-triones (3a-3j) were synthesized by condensation of aromatic aldehydes (1) and barbituric acid (2) in aqueous medium in presence of Amberlite IR-120 H catalyst. The structures of the synthesized compounds were confirmed by FT-IR, 1H NMR and mass spectroscopic studies. All the synthesized compounds were subjected to anti tuberculosis screening and molecular docking studies. Among them 3a and 3i were found to possess broad-spectrum antituberculosis activity and all the synthesized compounds are found to be promising potential specific inhibitors under molecular docking studies.